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Hubbard Center for Genome Studies

Linking Genomics to Organisms and the Environment
 
 

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Mexican tetra (Astyanax mexicanus) BAC Resources

Perhaps best known for its eyeless form (blind cavefish), the Mexican tetra is used as a model for studying the evolution of developmental pathways associated with adaptation to subterranean habitats. With support from the National Science Foundation (IBN #0208343) the HCGS has constructed a BAC library from a surface form of Astyanax.

The fish were supplied by Dr. Richard Borowsky (Dept. of Biology, New York University). The sibship (ID=Asty04, hatched 4/29/02) consisted of offspring of wild-caught parents collected from the Arroyo Sarco in january 2002, at the village of Encino, Tamaulipas, Mexico (Rio Sabinas drainage). The primary locality reference is R.M. Darnell, 1962. Fishes of the Rio Tamesi and Related Coastal Lagoons in East-Central Mexico. Publications of the Institute of Marine Science, Port Aransas, Texas Vol 8. ( DOC ).

Library availability

Plates and filters for the library are available from the HCGS.

Astyanax library

Segments Source Vector Cloning Site # clones Avg. insert size # plates # filters Genome coverage
A muscle pCC1BAC HindIII 58,752 104 153 3 3.6x
B muscle pCC1BAC - - - - - in preparation

Pricing

Library Segment Plates Price Filters Price
Astyanax library Segment A HCGS-01AM-AP $3,442 HCGS-01AM-AF $450.00
Astyanax library Segment B in prep - in prep -

Contact Dr. Thomas D. Kocher for more information.


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