![]() | ![]() |
| Hubbard Center for Genome Studies | |
| Linking Genomics to Organisms and the Environment | |
Perhaps best known for its eyeless form (blind cavefish), the Mexican tetra is used as a model for studying the evolution of developmental pathways associated with adaptation to subterranean habitats. With support from the National Science Foundation (IBN #0208343) the HCGS has constructed a BAC library from a surface form of Astyanax.
The fish were supplied by Dr. Richard Borowsky (Dept. of Biology, New York University). The sibship (ID=Asty04, hatched 4/29/02) consisted of offspring of wild-caught parents collected from the Arroyo Sarco in january 2002, at the village of Encino, Tamaulipas, Mexico (Rio Sabinas drainage). The primary locality reference is R.M. Darnell, 1962. Fishes of the Rio Tamesi and Related Coastal Lagoons in East-Central Mexico. Publications of the Institute of Marine Science, Port Aransas, Texas Vol 8. ( DOC ).
Plates and filters for the library are available from the HCGS.
| Segments | Source | Vector | Cloning Site | # clones | Avg. insert size | # plates | # filters | Genome coverage |
| A | muscle | pCC1BAC | HindIII | 58,752 | 104 | 153 | 3 | 3.6x |
| B | muscle | pCC1BAC | - | - | - | - | - | in preparation |
| Library Segment | Plates | Price | Filters | Price | |
| Astyanax library Segment A | HCGS-01AM-AP | $3,442 | HCGS-01AM-AF | $450.00 | |
| Astyanax library Segment B | in prep | - | in prep | - |
Contact Dr. Thomas D. Kocher for more information.
| Copyright ©2002, HCGS and Univ. of New Hampshire | |