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| Hubbard Center for Genome Studies | |
| Linking Genomics to Organisms and the Environment | |
Revised 7/29/05
To officially place an order please do the following:
1) Complete the HCGS order form (PDF, see details below).
2) Sign the UBMTA Implementing Letter PDF or the regular MTA).
3) FAX the completed HCGS order form and UBMTA implementing letter to HCGS, attention Krys Morris (603-862-2940).
PLEASE NOTE: Orders cannot be shipped if order forms are incomplete or if they are not accompanied by the appropriate Material Transfer Agreement. A UBMTA implementing letter is only required with the first order.
Order form: To order library plates, high-density hybridization filters or individual clone stocks, complete the HCGS order form with all required information and fax it to the attention of Krys Morris (603-862-2940). The order form can be downloaded as a PDF.
Material Transfer Agreement: All of our library resources are freely available upon completion of a Material Transfer Agreement. Several hundred academic institutions and private organizations (click here for list) have adopted and signed a master Universal Biological Materials Transfer Agreement (UBMTA) promoted by the National Institutes of Health (NIH). If your institution has already signed a UBMTA, only the UBMTA Implementing Letter must be completed and returned to us.
If your institution has not signed a UBMTA, a regular Material Transfer Agreement (MTA) must be completed. An example of this agreement is available here (regular MTA). More information on Material Transfer Agreements can be found on the www site of the UNH Office of Intellectual Property Management (OIPM). This booklet provides a useful overview of the reasons why MTAs are needed. Should you require additional information or have specific questions about the MTA, please contact Paula Pelletier, Office of Intellectual Property Management, (paula.pelletier@unh.edu).
Our BAC and cDNA libraries are available as library plates (microtiter dishes 384-well), high-density colony hybridization filters and as single clone stocks.Specific information about each library (DNA source, redundancy, average insert size etc.) can be obtained by following the link above.
High Density Filters: All of our libraries are arrayed onto 22x22cm positively charged nylon filters for hybridization-based screening. Each filter contains 18,432 independent clones spotted in duplicate in a 4x4 array pattern for a total of 36,864 colonies. Filters can be screened with 32P (random primed) probes, overgo probes and enhanced chemiluminescence (ECL). BAC library filters are available as 3-5X coverage sets. Filters sets will be accompanied by complete documentation on how to interpret hybridization results including necessary overlays for locating positive clones on the autorad image of the probed filter (please follow the links above for more details on filter interpretation and vector data).
Individual BAC and cDNA clones: Individual clones are available as bacterial agar stabs in the appropriate host strain.
Library Plates: All libraries are arrayed in 384-well microtiter plates. Clones in each well are stored in freezing buffer and the appropriate antibiotic. BAC library plates are available as segment sets, each segment representing a 3-5X genome equivalent (please refer to our BAC resources web page).
Our prices are calculated strictly on a cost recovery basis. A list of services and our fee schedule can be found here: HCGS rates.pdf
High density filters: Filters are available at a cost of US$150.00 per filter. Please note that BAC library filters are only available as 3-5x coverage sets at a cost of US$150.00 for each filter in the set. Individual cDNA filters and BAC filter sets will be accompanied by complete documentation on how to read and interpret the hybridization results.
Individual BAC clones: A set up fee of US$35 will be charged with each order. Clones# 1-20: US$20 each. Clones# 21-40: US$16 each. Clones# 41 and above: US$12.50 each.
Library plates: BAC library plates are available as 3-5x coverage segments. The price is US$22.50/plate.
Shipping costs: We prefer to bill shipping costs to the recipient's courier account number. If the recipient's institution does not have a courier account number, we will add shipping costs to the final invoice. Shipping cost will vary depending on item ordered, final destination and where applicable custom regulations. Our preferred courier, where possible, is Fedex. We will inform you if a different company must be used.
High density filters: Filters are normally in stock and will be shipped upon receipt of order. If filters are out of stock you will be notified and given an estimated date of delivery.
Individual BAC and cDNA clones: Requests for agar stabs will normally be processed within one week of receipt of order.
Library plates: Due to the time and logistics involved in the duplication and shipping process, orders should be made well in advance. Libraries in stock will normally be shipped within two weeks of receipt of order. If out of stock, expect a delay of 1-2 months, as we try to avoid repeated freeze thaw cycles of the master plates.
| Copyright ©2002, HCGS and Univ. of New Hampshire |  |