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| Hubbard Center for Genome Studies |
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| Linking Genomics to Organisms and the Environment |
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[ HCGS home | Libraries available| Ordering information| Protocols| Filter interpretation| Vector data]Tilapia (Oreochromis niloticus) BAC Resources
Tilapia (Oreochromis spp.) are one of the most important species in aquaculture today, with world-wide production exceeding 1 billion pounds per year. Increasingly popular in western cuisine, tilapia are a particularly important source of protein in less developed countries.
Four tilapia BAC libraries have been produced at the Tokyo University of Fisheries from the sperm of a single fish (individual # 00-0135-EA1B). This fish is part of a stock originally collected from Lake Manzallah (Egypt), and maintained at the University of Stirling, UK (Katagiri et al. 2001) [PDF].
| Library | # primary clones | Average insert size | Estimated coverage | Picked |
| 1 | 120k | 65k | 6x | - |
| 2 | 800k | 105k | 65x | - |
| 3 | 100k | 145k | 11x | 36k |
| 4 | 40k | 194k | 6x | 33k |
With support from the US Dept. of Agriculture, we have fingerprinted more than 35,000 clones from libraries 3 and 4 (approximately 5x coverage of the genome). The assembled contigs can be viewed on our HCGS FPC viewer.
Library availability
Plates and filters for portions of libraries 3 and 4 are available from the HCGS.
Tilapia library #3
| Segments | Source | Vector | Cloning Site | # clones | Avg. insert size | # plates | # filters | Genome coverage |
| A (fingerprinted) | sperm | pBAC-lac | HindIII | 18,432 | 145kb | 48 | 1 | 2.5x |
| B | sperm | pBAC-lac | HindIII | 18,432 | 145kb | 48 | 1 | 2.5x |
Tilapia library #4
| Segments | Source | Vector | Cloning Site | # clones | Avg. insert size | # plates | # filters | Genome coverage |
| A (fingerprinted) | sperm | pBAC-lac | HindIII | 18,432 | 194kb | 48 | 1 | 3.3x |
| B | sperm | pBAC-lac | HindIII | 14,976 | 194kb | 39 | 1 | 2.7x |
Pricing
| Library Segment | Plates | Price | | Filters | Price |
| Tilapia library 3 Segment A | HCGS-03TI-AP | $960.00 | | HCGS-03TI-AF | $150.00 |
| Tilapia library 3 Segment B | HCGS-03TI-BP | $960.00 | | HCGS-03TI-BF | $150.00 |
| Tilapia library 4 Segment A | HCGS-04TI-AP | $960.00 | | HCGS-04TI-AF | $150.00 |
| Tilapia library 4 Segment B | HCGS-04TI-BP | $780.00 | | HCGS-04TI-BF | $150.00 |
Contact Dr. Thomas D. Kocher for more information.
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