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Hubbard Center for Genome Studies

Linking Genomics to Organisms and the Environment
 
 

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Tilapia (Oreochromis niloticus) BAC Resources

Tilapia (Oreochromis spp.) are one of the most important species in aquaculture today, with world-wide production exceeding 1 billion pounds per year. Increasingly popular in western cuisine, tilapia are a particularly important source of protein in less developed countries.

Four tilapia BAC libraries have been produced at the Tokyo University of Fisheries from the sperm of a single fish (individual # 00-0135-EA1B). This fish is part of a stock originally collected from Lake Manzallah (Egypt), and maintained at the University of Stirling, UK (Katagiri et al. 2001) [PDF].

Library # primary clones Average insert size Estimated coverage Picked
1 120k 65k 6x -
2 800k 105k 65x -
3 100k 145k 11x 36k
4 40k 194k 6x 33k

With support from the US Dept. of Agriculture, we have fingerprinted more than 35,000 clones from libraries 3 and 4 (approximately 5x coverage of the genome). The assembled contigs can be viewed on our HCGS FPC viewer.

Library availability

Plates and filters for portions of libraries 3 and 4 are available from the HCGS.

Tilapia library #3

Segments Source Vector Cloning Site # clones Avg. insert size # plates # filters Genome coverage
A (fingerprinted) sperm pBAC-lac HindIII 18,432 145kb 48 1 2.5x
B sperm pBAC-lac HindIII 18,432 145kb 48 1 2.5x

Tilapia library #4

Segments Source Vector Cloning Site # clones Avg. insert size # plates # filters Genome coverage
A (fingerprinted) sperm pBAC-lac HindIII 18,432 194kb 48 1 3.3x
B sperm pBAC-lac HindIII 14,976 194kb 39 1 2.7x

Pricing

Library Segment Plates Price Filters Price
Tilapia library 3 Segment A HCGS-03TI-AP $960.00 HCGS-03TI-AF $150.00
Tilapia library 3 Segment B HCGS-03TI-BP $960.00 HCGS-03TI-BF $150.00
Tilapia library 4 Segment A HCGS-04TI-AP $960.00 HCGS-04TI-AF $150.00
Tilapia library 4 Segment B HCGS-04TI-BP $780.00 HCGS-04TI-BF $150.00

Contact Dr. Thomas D. Kocher for more information.


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