Lectures: MWF 10:10-11:00AM, Iddles L103
Instructors: John Collins, Department of Biochemistry,304 Rudman Hall (collins@genome.unh.edu) Tom Kocher, Departmentof Zoology, 216 Rudman Hall (tdk@cisunix.unh.edu)
Office hours: By appointment Teaching Assistants: CheryPugh (capugh@cisunix.unh.edu), Jeff McMahon (jsmm@cisunix.unh.edu),Nancy Garnhart (garnhart@cisunix.unh.edu)
Recitation: 1 hour each week
Sec. 1 - Tuesday 10-11 Kingsbury 251 (Note change from Time& Room Schedule!)
Sec. 2 - Wednesday 8- 9 Rudman G17
Sec. 3 - Tuesday 2- 3 Rudman G17
Sec. 4 - Friday 12- 1 Rudman G17
Sec. 5 - Wednesday 1- 2 SLS G16
Sec. 6 - Thursday 10-11 SLS 220
Text: Hartwell et al., "Genetics: From Genes toGenomes" 4th edition, McGraw Hill (1st edition)
Prerequisites: Biol 411 and 412, Chem 403 and 404
Evaluation: Four "hour exams" and a finalwill be given. Your best 3 scores on the hour exams will countfor a total of 50% of your grade. Makeups normally will not begiven - if you miss an exam we will count the other 3 exams. Thefinal will count for 25% of your grade, and will cover the entiresemester. Weekly 10 minute quizzes in recitation will contributethe remaining 25% of your grade.
Dates Topics Readings
Jan 19 Course structure and goals Preface, Prologue
Jan 21-26 Principles of genetic transmission Chapter 1
Segregation and assortment
Probability and dominance
Pedigree analysis
Jan 28-31 Genotypes and Phenotypes Chapter 2
Incomplete dominance, multiple alleles
Genes, enzymes and complementation
Feb 2-4 Genes and chromosomes Chapter 3
Mitosis and meiosis
Sex chromosomes
Feb 7-11 Linkage and mapping Chapter 4
Chi-square and identification of linkage
Three-point crosses
Maps and map distances
Feb 14-21 DNA structure and replication Chapter 5
DNA is the genetic material
Replication
Recombination
Feb 23-28 Mutational analysis Chapter 6
Molecular basis of mutation
Mechanisms of DNA repair
Genes and proteins
Mar 1-6 Gene expression Chapter 7
The genetic code
Transcription
Translation
Mar 8 Genetic analysis in C. elegans Chapter 20
Mar 20-24 Genetic engineering Chapter 8
Restriction enzymes and cloning vectors
Genomic and cDNA libraries, screening
PCR and DNA sequencing
Mar 27-29 Molecular polymorphisms Chapter 9
Methods in DNA testing
Social and ethical issues
Mar 31-Apr 5 Analysis of large genomes Chapter 10
Genetic and physical maps
Positional cloning and candidate genes
Mapping of quantitative traits
Apr 7-14 Eukaryotic chromosomes Chapters 11-12
Nucleosomes, centromeres and telomeres
Chomatin packaging and gene expression
Inversions and polyploidy
Apr 17-19 Genetic analysis in bacteria Chapter 13
Mapping by conjugation
Transduction mapping
Apr 21-24 Gene regulation in prokaryotes Chapter 15
The Lac operon
Lysis or lysogeny?
Apr 26-28 Gene regulation in eukaryotes Chapter 16
DNA rearrangements and gene regulation
Combinatorial control of eucaryotic transcription
May 3-5 Population genetics Chapter 23
Hardy-Weinberg and the four forces
Natural selection and evolution
May 8 Molecular evolution Chapter 24
Last Lecture: Monday, May 8