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Microsatellite library
Introduction

Enrichment
Extract DNA
Digest DNA
Size selection
Ligate linkers
PCR amplify #1
Enrichment
PCR amplify #2
TA cloning

Cloning and picking
Overview
Transformation
Picking
PCR test
Miniprep
Sequencing

Primer design
Primer design
Primer testing
Genbank submission

05/03