General Sample Submission Requirements
Samples must be submitted in properly sealed and clearly labeled tubes or, preferably, 96-well PCR plates.
Samples should be a standard volume and concentration (quantified using fluorescence-based assays), which are determined based on the specific library being requested (see Specific Sample Submission Requirements below).
Sample submission must be approved prior to the samples being sent to HCGS (email confirmation) and preceded by an Excel sample sheet with a list of sample names, tube/well identifiers, concentrations, volumes and project names.
Also included in the service request email should be (1) a brief description of the project, (2) total number of samples, (3) list of requested services, (4) special request or handling/care instructions, (5) names of the PI(s) and (6) appropriate identifiers for grants and/or other funding sources.
Please fill out the HCGS Service Request Form prior to sending samples.
Sample Volume and Concentration Requirements
- DNA for Amplicon - A concentration between 1ng/ul and 10ng/ul in a volume of 20ul.
- PCR Product for Indexing - 4ul of PCR product diluted in 36ul of nuclease-free water for a total of 40ul of diluted product.
- DNA for Illumina WGS/Metagenomic/Plasmid - A concentration of 1ng/ul in a volume of 10ul.
- Total RNA for Illumina RNASeq - Between 100ng and 1,000ng total in a volume of 50ul.
- DNA for PacBio WGS - Between 300ng and 1,000ng total in a volume of 50ul.
- Total RNA for PacBio Kinnex Between 100ng and 300ng total in a volume of 20ul.
- DNA for GBS - 20ng/ul in 20ul.
- Olink - Contact us.
Shipping Instructions
- Only use FedEx or UPS (USPS may not get to the correct location)
- Only ship samples on Monday, Tuesday or Wednesday.
- Please use the following shipping address:
Hubbard Center for Genome Studies
Attn: Kelley Thomas
35 Colovos Road
447 Gregg Hall
Durham, NH 03824
603.862.2470
