Current Pricing Guidelines
We provide consultation on sample preparation and pooling for the best use of the NovaSeq and HiSeq sequencers.
The following table summarizes the current pricing guidelines.
| Service | FY23 | Unit |
|---|---|---|
| NovaSeq SP PE 250 | $3,034.00 | Lane |
| NovaSeq SP PE 150 | $2,419.00 | Lane |
| NovaSeq SP PE 100 | $2,291.00 | Lane |
| NovaSeq SP PE 50 | $1,958.00 | Lane |
| Qubit HS-DNA | $ 5.10 | sample |
| QubitRNA | $5.60 | sample |
| Sample pooling/normalizaton | $74.00 | pool (up to 96) |
| KapaRNA Hyperprep | $147.00 | library |
| Kapa HyperPlus DNA library | $69.00 | library |
| Kapa Hyperplus DNA library (dilute) | $42.00 | library |
| Genotyping by sequencing (GBS Libraries per plate) | $778.00 | plate (96) |
| Amplicon Libraries (adapter only) | $262.00 | plate (96) |
| Amplicon Libraries (two step) | $490.00 | plate (96) |
| Bioinformatics Services | $106.00 | hour |
| Lab Services | $51.00 | hour |
| Kapa Hyperprep with riboerase (HMR) | $212.00 | library |
Request Services
To request services, please fill out the form below. If you have any questions or would like to discuss your project, please email unh.hcgs@unh.edu.
General Sample Submission Requirements
Samples must be submitted in properly sealed and clearly labeled tubes or, preferably, 96-well PCR plates.
Samples should be a standard volume and concentration (quantified using fluorescence-based assays), which are determined based on the specific library being requested (see Specific Sample Submission Requirements below).
Sample submission must be approved prior to the samples being sent to HCGS (email confirmation) and preceded by an Excel sample sheet with a list of sample names, tube/well identifiers, concentrations, volumes and project names.
Also included in the service request email should be (1) a brief description of the project, (2) total number of samples, (3) list of requested services, (4) special request or handling/care instructions, (5) names of the PI(s) and (6) appropriate identifiers for grants and/or other funding sources.
Please fill out the HCGS Service Request Form prior to sending samples.
Shipping Instructions
- Only use FedEx or UPS (USPS may not get to the correct location)
- Only ship samples on Monday, Tuesday or Wednesday.
- Ensure you've been contacted by us and we have confirmed that the samples may be shipped.
- Please use the following shipping address:
Hubbard Center for Genome Studies
35 Colovos Road 447
Gregg Hall Durham, NH
03824 603.862.2470
Library Specific Sample Submission Requirements
NOTE: Volumes are based on the recommend input for the library preps described, however, providing double the suggested volume is recommended.
Illumina TruSeq PCR-free DNA Library
For a 550bp insert size, 2ug of gDNA in a total volume of 55ul is recommended
For a 350bp insert size, 1ug of gDNA in a total volume of 55ul is recommended
Illumina TruSeq Stranded RNA Library
0.1 – 1.0ug of total RNA in a total volume of 10ul
Illumina Nextera Standard Library
50ng of gDNA in a total volume of 20ul
Illumina Nextera Dilute Library
gDNA should be sent at a concentration of 2.5ng/ul in a total volume of 10ul
Illumina Mate Pair Library
Gel-plus protocol, 4ug in a total volume of 200ul
Gel-free protocol, 1ug in a total volume of 50ul
Illumina Chip Seq
5 – 10ng of ChIP-enriched, fragmented DNA in a total volume of 50ul
Genotyping by Sequencing (GBS)
200ng of gDNA in a total volume of 10ul
Samples must be quantified using a fluorometric dye (e.g. Qubit) and at the correct concentration and volume (10ul of 20ng/ul) for processing.
If submitting more than 8 samples, then samples must be in a 96 well format (either strip tubes or PCR plate(s))
Earth Microbiome Amplicon Libraries (16S/18S/ITS, etc)
gDNA should be sent at a concentration of 2.5ng/ul in a total volume of 10ul
